A spectrophotometer is a device used to measure the intensity of light as a function of its wavelength. The device consists of a light source, a monochromator, and a detector. The light source is usually a lamp or laser. The monochromator is used to select a specific wavelength of light, and the detector measures the intensity of the selected wavelength. Spectrophotometers can be used to measure the concentrations of dilute solutions and can also be used to detect substances in the solid state. Spectrophotometers are a commonly used instrument in science, medicine, and many other fields.
How does a spectrophotometer work ?
Spectrophotometers can measure the concentrations of dilute solutions and can be used to determine substances in the solid state. This device includes a single light source, a monochromator, and a detector.
Spectrophotometer working step-1 : Light is produced using a single light source. As light hits the diffuser, the grating, which produces a prism effect, separates the beam into its component colors. It is then rotated until a selected wavelength reaches an exit slit, allowing it to be transmitted in line with the named sample. The light then interacts with a sample.
Spectrophotometer Working Step -2 :
After interacting of light with the sample, The detector measures the amount of light that passes through completely through the sample and hits the detector (transmittance) and measurement of the amount of light that is absorbed by a sample (absorbance) of the sample. The detector interprets the light that passes through a sample as a voltage signal. The light that is passed through the sample is detected by the detector, which converts the content into a digital readout.
Different Types of Spectrophotometer :
There are several types of spectrophotometer, and it's important to know which one best suits your needs when looking for the right spectrophotometer for you. Here's a guide to help you understand the types of spectrophotometers available in the market.
UV Visible Spectrophotometer :
These spectrophotometers use a light source to illuminate a sample with light across the ultraviolet to the visible wavelength range (typically 190 to 900 nm). The instruments then measure the light absorbed, transmitted, or reflected by the sample at each wavelength.
Atomic Absorption Spectrophotometer :
Atomic absorption spectrometry analyzes the concentration of chemical elements in a sample by absorbing certain wavelengths of visible light from atoms in the sample. These spectrometers typically include a flame burner to atomize the sample, a monochromator, and a detector that detects light.
Single Beam Spectrophotometer :
These spectrophotometers use a single source of light.
Double Beam Spectrophotometer :
Double-beam spectrophotometer uses the light source and then splits the light into two separate parts.
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